Receptor Dynamics

Bünemann Lab

phone: +49 (0)6421-28-25773 email: moritz.buenemann@staff.uni-marburg.de

We study spatio-temporal signaling mechanisms and kinetics of G protein-coupled receptor (GPCR) systems in living cells.
Research
  • Kinetics of receptor activation, receptor/G protein interaction, G protein activation , G protein/effector interaction
  • Distribution of receptors G proteins and effectors into membrane microdomains and their impact on dynamics of GPCR signaling
  • Voltage dependence of receptor activation
  • Regulation of receptor function by arrestin

Imaging of G protein signalling in intact tissues

  • Real time cAMP imaging by means of FRET in tissues from transgenic mice
  • Generation of mice with tissue specific expression of FRET-based biosensors

Experimental techniques

  • Fluorescence microscopy
  • Förster (fluorescence) resonance energy transfer (FRET)
  • Total internal reflection microscopy (TIRF)
  • Patch clamp recording
  • Cell culture and transfection
  • Molecular biology, cloning
  • Immunhistochemistry, immunoblots, immunprecipitation, confocal laser scanning microscopy
  • RNAi-technology
Selected Publications
  • Dynamics of Gαq-protein-p63RhoGEF interaction and its regulation by RGS2.
    Bodmann EL, Rinne A, Brandt D, Lutz S, Wieland T, Grosse R, Bünemann M.
    Biochem J. 2013 Dec 4. [Epub ahead of print]
  • Voltage regulates adrenergic receptor function.
    Rinne A, Birk A, Bünemann M.
    Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1536-41.
  • A polymorphism-specific “memory” mechanism in the β(2)-adrenergic receptor.
    Ahles A, Rochais F, Frambach T, Bünemann M, Engelhardt S.
    Sci Signal. 2011 Aug 9;4(185):ra53. doi: 10.1126/scisignal.2001681.
  • Analysis of receptor oligomerization by FRAP microscopy.
    Dorsch S, Klotz KN, Engelhardt S, Lohse MJ, Bünemann M.
    Nat Methods. 2009 Mar;6(3):225-30.
  • Real-time optical recording of beta1-adrenergic receptor activation reveals supersensitivity of the Arg389 variant to carvedilol.
    Rochais F, Vilardaga JP, Nikolaev VO, Bünemann M, Lohse MJ, Engelhardt S.
    J Clin Invest. 2007 Jan;117(1):229-35.
  • Dynamics of receptor/G protein coupling in living cells.
    Hein P, Frank M, Hoffmann C, Lohse MJ, Bünemann M.
    EMBO J. 2005 Dec 7;24(23):4106-14.